Advantages:

• Laser assisted hatching takes less time and does not expose the embryos to potentially adverse chemicals, so embryos spend less time out of optimal culture conditions.
• It also requires significantly less physical manipulation of the embryos.
• The cumulative effect of these factors is to minimize the stress places upon the embryos during the performance of assisted hatching. This in turn should then translate into improved implantation and pregnancy rates.

Principle of Laser:-

• Zona drilling of embryos would be performed with the use of UV laser microbeam. The embryos will be placed under mineral oil within 20 microlitre droplet of IVF culture medium
• Minute laser focus adjustment will performed with the laser interface
• Appropriate laser energy to be selected so that every single laser shot revealed a small visible impact within the zona.
• Pulse energy of 2-5 mJ at the object plane and a pulse repetition rate of 15-20 pulses will be convenient for the procedure.

Process:-

• Infra red diode laser light focused through a microscope objective has enabled rapid non-touch micro drilling.
• Embryos are maintained in their culture medium. Special care should be taken to open the internal side of the zona.
• No washing of the embryos is needed after laser drilling.
• This procedure appears to be quick, precise and is chemical free.